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GE Healthcare - 17-5156-01 - HiTrap Q FF

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Manufacturer: GE Healthcare
Item #: 3527826
Mfr Part Number: 17-5156-01
MSRP: unlisted
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HiTrap Q FF is prepacked with Q Sepharose Fast Flow, and is a strong anion exchanger for small-scale protein purifications as well as screening of binding and elutions conditions. Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.

The industry standard for ion exchange chromatography. High flow rates and good scale-up potential. Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient. Predictable scale-up Q, SP, DEAE, and CM Sepharose Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities.

ANX Sepharose 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins. Bed Volume: 5 ml Storage Conditions: 4 to 30C, 20% Ethanol Pressure Max.

[Over the Packed Bed During Operation]: 5 bar [0.5 MPa] (70 psi) Application: For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules BioProcess resin: Yes Ligand: Quaternary amine Particle size, d50V: ~90 m1) Ion Exchanger Type: Strong anion exchanger Matrix: cross-linked, 6% agarose particles Ionic Capacity: 0.

18-0.24 mmol Cl-/ml medium PH stability, operational: 2-122) PH stability, CIP: 2-143) PH ligand fully charged: Entire pH range4) Pressure/Flow Specification: 400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm Exclusion Limit [Mr] [Globular Proteins]: ~4 106 Storage: 4 to 30C, 20% Ethanol Chemical Stability: Stable to commonly used aqueous buffers:1.

0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol5) Certificate of Analysis: Yes Column i.d.: 16 mm Bed Dimensions: 16 25 mm Complete Packsize: 5 ml Material [Column Hardware]: Polypropylene (PP).



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