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Industrial And Scientific > Laboratory And Science > Chemical Solutions > Reagents > Drug Discovery Reagents > PerkinElmer

PerkinElmer - AL3024F - Lambda Light Chain (human) AlphaLISA Detection Kit, 5, 000 Assay Points

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Spacer PerkinElmer - AL3024F - Lambda light chain (human) AlphaLISA Detection Kit, 5, 000 assay points Spacer
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Manufacturer: PerkinElmer
Item #: 10483887
Mfr Part Number: AL3024F
MSRP: $16,467.68
Sale Price: Click for Price

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The AlphaLISA immunoassay kit for human lambda light chain enables the quantitative determination of human light chain in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). Formats: Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 micro L reaction volume (10 micro L of sample).

Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 micro L reaction volume (5 micro L of sample). Our 5, 000 assay point kit allows you to run 5, 000 wells in 96-well or 384-well format, using a 50 micro L reaction volume (5 micro L of sample).

Features: No-wash steps, no separation steps ELISA alternative technology Sensitive detection Broad sample compatibility Small sample volume Results in less than 3 hours Half the time of an ELISA assay AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode.

In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity.

The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. Most mammal IgG class antibodies, including those from human are composed of two heavy and two light chains.

Such light chains can be of kappa or lambda types, with an expected ratio of 3 kappas to one lambda. Both appear to play the same function in antibodies. Free light chains are usually rare in blood. However, several types of multiple myelomas will secrete free light chains, which can then accumulate in organs such as the kidney and cause severe damage, with lambda chains being the poorest diagnostic.

Knowledge of the type of light chain generated is also important for the creation and production of therapeutic antibodies. Techniques used for purification, such as protein L or anti species antibodies, can have different affinities for the two chains.

The lambda light chain AlphaLISA detection kit allows for the detection of lambda light chain in human sera, plasma, and cell culture supernatants. Disclaimer: For research use only. Not for use in diagnostic procedures. Assay Target: Lambda light chain Assay Target Class: Antibody Automation Compatible: Yes Detection Method: Alpha Product Brand Name: AlphaLISA Shipping Condition: Blue Ice Target Species: Human Therapeutic Area: Biologics/Bioprocess Unit Size: 5, 000 Assay Points.





Since 2002, Neobits has made the full range of products in telecom, IT, AV, lab supplies and more, like this PerkinElmer - AL3024F - Lambda Light Chain (human) AlphaLISA Detection Kit, 5, 000 Assay Points, available to individulals, agencies and businesses alike. Please add item to your cart and supply your zipcode for exact shipping options and charges. Right now, our price of $15,683.50 is 5% off the typical list price - a savings of $784.18! You may complete your purchase online with any major credit card or call us anytime at 1-866-636-2487 with questions or purchase needs. To request a phone system quote or to get info on the most suitable system for your operation, Click Here.


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