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Industrial And Scientific > Laboratory And Science > Chemical Solutions > Reagents > Drug Discovery Reagents > PerkinElmer

PerkinElmer - AL3040HV - TSLP (human) AlphaLISA Detection Kit, 100 Assay Points

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Manufacturer: PerkinElmer
Item #: 10260057
Mfr Part Number: AL3040HV
MSRP: $688.11
Sale Price: Click for Price

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The AlphaLISA immunoassay kit for human TSLP enables the quantitative determination of human TSLP in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). Formats: Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 micro L reaction volume (10 micro L of sample).

Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 micro L reaction volume (5 micro L of sample). Our 5, 000 assay point kit allows you to run 5, 000 wells in 96-well or 384-well format, using a 50 micro L reaction volume (5 micro L of sample).

Features: No-wash steps, no separation steps ELISA alternative technology Sensitive detection Broad sample compatibility Small sample volume Results in less than 3 hours Half the time of an ELISA assay AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode.

In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity.

The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. Thymic stromal lymphopoietin (TSLP) is a cytokine that plays a critical role in the maturation of T cells through antigen presenting cells.

TSLP is mainly produced by epithelial cells and its expression is regulated by many other cytokine such as IL1 and TNF. TSLP expression is linked to many disease states such as asthma, inflammatory arthritis, atopic dermatitis, eczema, eosinophilic esophagitis and other allergic states.

Thus, TSLP becomes one of many important biomarkers for airway inflammatory diseases. This kit is designated to detect and quantify the levels of TSLP in cell culture media and other biological matrices. Disclaimer: For research use only. Not for use in diagnostic procedures.

Assay Target: TSLP Assay Target Class: Cytokine Automation Compatible: Yes Detection Method: Alpha Product Brand Name: AlphaLISA Shipping Condition: Blue Ice Target Species: Human Therapeutic Area: Cancer Unit Size: 100 Assay Points.



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