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PerkinElmer - AL565C - IL-13 (mouse) AlphaLISA Detection Kit, 500 Assay Points

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Manufacturer: PerkinElmer
Item #: 10260059
Mfr Part Number: AL565C
MSRP: $2,058.15
Sale Price: Click for Price

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The AlphaLISA immunoassay kit for mouse IL-13 enables the quantitative determination of mouse interleukin-13 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). Formats: Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 micro L reaction volume (10 micro L of sample).

Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 micro L reaction volume (5 micro L of sample). Our 5, 000 assay point kit allows you to run 5, 000 wells in 96-well or 384-well format, using a 50 micro L reaction volume (5 micro L of sample).

Features: No-wash steps, no separation steps ELISA alternative technology Sensitive detection Broad sample compatibility Small sample volume Results in less than 3 hours Half the time of an ELISA assay AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode.

In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity.

The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. Interleukin 13 (IL13) is a 12 kDa cytokine produced by activated Th2 cells and involved in several stages of B-cell maturation and differentiation.

It up-regulates CD23 and MHC class II expression. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. It induces many symptoms of lung allergic disease, including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion, which all contribute to airway obstruction.

Disclaimer: For research use only. Not for use in diagnostic procedures. Assay Target: IL13 Assay Target Class: Cytokine Automation Compatible: Yes Detection Method: Alpha Product Brand Name: AlphaLISA Shipping Condition: Dry Ice Target Species: Mouse Unit Size: 500 Assay Points.





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