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Industrial And Scientific > Laboratory And Science > Chemical Solutions > Reagents > Drug Discovery Reagents > PerkinElmer

PerkinElmer - AL570F - IL-25 (mouse) AlphaLISA Detection Kit, 5, 000 Assay Points

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Manufacturer: PerkinElmer
Item #: 10483892
Mfr Part Number: AL570F
MSRP: $16,467.68
Sale Price: Click for Price

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The AlphaLISA immunoassay kit for mouse IL-25 enables the quantitative determination of mouse interleukin-25 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). Formats: Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 micro L reaction volume (10 micro L of sample).

Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 micro L reaction volume (5 micro L of sample). Our 5, 000 assay point kit allows you to run 5, 000 wells in 96-well or 384-well format, using a 50 micro L reaction volume (5 micro L of sample).

Features: No-wash steps, no separation steps ELISA alternative technology Sensitive detection Broad sample compatibility Small sample volume Results in less than 3 hours Half the time of an ELISA assay AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode.

In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity.

The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. Interleukin 25 (IL-25), also known as interleukin 17E is a member of the IL-17 family that is secreted by lymphocytes to stimulate the production of IL-4, IL-5 and IL-13, all mediators of inflammation.

IL-25 is mainly expressed by cells in the digestive system and plays a major role in immunity of the gastro-intestinal track. However, it is also involved in inflammation of those tissues. IL-25 is also known as an anti-tumor agent in these tissues, preventing colon, pancreatic and liver cancer.

This kit allows for the detection of IL-25 in biological samples. Disclaimer: For research use only. Not for use in diagnostic procedures. Assay Target: IL25 Assay Target Class: Cytokine Automation Compatible: Yes Detection Method: Alpha Product Brand Name: AlphaLISA Shipping Condition: Blue Ice Target Species: Mouse Therapeutic Area: Inflammation Unit Size: 5, 000 Assay Points.





Looking for PerkinElmer - AL570F - IL-25 (mouse) AlphaLISA Detection Kit, 5, 000 Assay Points at an unbelieveable price? Look no further than Neobits.com! In-stock items usually ship within one business day of payment receipt. We do not ship on weekends. This PerkinElmer - AL570F - IL-25 (mouse) AlphaLISA Detection Kit, 5, 000 Assay Points is currently on sale for $15,683.50, a discount of 5% off the list cost. You can complete your purchase via credit card with your valid Visa, Mastercard, Discover or AmericanExpress card or use your PayPal or Google Checkout account. In order to most efficiently serve you, credit card transactions and order fulfillment are handled by established third party banking, processing agents and distribution institutions.


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